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ATJ01

ATJ01

Jo-1

Native - Calf Thymus
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Histidyl tRNA synthetase
HisRS
Histidine tRNA ligase
Uniprot ID:  Q2KI84
mRNA RefSeq:  NM_001046066
Protein RefSeq:  NP_001039531
Polymyositosis (PM) and dermatomyositis (DM) are idiopathic inflammatory myopathies. The existence of autoantibodies to nuclear and cytoplasmic antigens, in particular to the aminoacyl-tRNA synthetase group of enzymes, in the sera of up to 89% of patients indicates that these diseases have an autoimmune origin. The most common autoantibody in PM and DM is anti Jo-1, occurring in 15-20% of all myositis patients and about 30% of adult PM patients.

Jo-1 autoantibodies are capable of inhibiting the activity of the target antigen (HRS) and can immunoprecipitate labelled enzyme as well as its specific tRNA. HRS has a molecular weight of 55 kDa in SDS-polyacrylamide electrophoresis, and is believed to exist as a dimer in its native state.

The amino acid sequence of human Jo-1 antigen has been determined by cloning and sequencing its cDNA. It contains three structural motifs typical of class IIa aminoacyl transferases and two signature regions common to histidyl-tRNA synthetases. The human enzyme amino acid sequence shows significant homology to those of the enzymes from yeast (47.5%) and E. coli. It is predicted to have a coiled-coil α-helical structure that is characteristic of many autoantigens and which contains the major autoantigenic epitope. 

 
PDF-logo-dl Jo-1 datasheet
Polymyositis
Dermatomyositis
Please log in to view certificates of analysis for this item
  • Targoff, I.N. (1991) Curr. Prob. Dermatol. 3, 134
  • Plotz, P.H. et al. (1989) Ann. Int. Med. 111, 143
  • Reichlin, M. et al. (1984) Arthritis Rheum. 27, 1150
  • Nishikai, M., Reichlin, M. (1980) Arthritis Rheum. 23, 881
  • Targoff, I.N. (1990) Rheum. Dis. Clin. North Am. 18, 455
  • Mathews, M.B., & Bernstein, R.M. (1983) Nature
  • 304, 177 Mirande, M. (1991) Prog. Nucl. Acid Res. 40, 95
  • Rosa, M.D. et al. (1983) Nucl. Acids Res. 11, 853
  • Biswas, T. et al. (1987) J. Immunol. Meth. 98, 235
  • Raben N. et al. (1991) Nucl. Acids Res. 20, 1075
  • Raben, N. et al. (1994) J. Biol. Chem. 269, 24277
  • Blechynden, L.M. et al. (1996) Gene 178, 151
  • Targoff, I.N., Reichlin, M. (1987) J. Immunol. 138, 2874
  • Walker, E.J. et al. (1987) J. Immunol. Meth. 96, 149
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AROTEC Diagnostics
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