icon lock Secure Shipping Worldwide
cart Shopping Cart Cart (0)

ATJ01

ATJ01

Jo-1

Native - Calf Thymus
Enquire
Histidyl tRNA synthetase
HisRS
Histidine tRNA ligase
Uniprot ID:  Q2KI84
mRNA RefSeq:  NM_001046066
Protein RefSeq:  NP_001039531
Polymyositosis (PM) and dermatomyositis (DM) are idiopathic inflammatory myopathies. The existence of autoantibodies to nuclear and cytoplasmic antigens, in particular to the aminoacyl-tRNA synthetase group of enzymes, in the sera of up to 89% of patients indicates that these diseases have an autoimmune origin. The most common autoantibody in PM and DM is anti Jo-1, occurring in 15-20% of all myositis patients and about 30% of adult PM patients.

Jo-1 autoantibodies are capable of inhibiting the activity of the target antigen (HRS) and can immunoprecipitate labelled enzyme as well as its specific tRNA. HRS has a molecular weight of 55 kDa in SDS-polyacrylamide electrophoresis, and is believed to exist as a dimer in its native state.

The amino acid sequence of human Jo-1 antigen has been determined by cloning and sequencing its cDNA. It contains three structural motifs typical of class IIa aminoacyl transferases and two signature regions common to histidyl-tRNA synthetases. The human enzyme amino acid sequence shows significant homology to those of the enzymes from yeast (47.5%) and E. coli. It is predicted to have a coiled-coil α-helical structure that is characteristic of many autoantigens and which contains the major autoantigenic epitope. 

 
PDF-logo-dl Jo-1 datasheet
Polymyositis
Dermatomyositis
Please log in to view certificates of analysis for this item
  • Targoff, I.N. (1991) Curr. Prob. Dermatol. 3, 134
  • Plotz, P.H. et al. (1989) Ann. Int. Med. 111, 143
  • Reichlin, M. et al. (1984) Arthritis Rheum. 27, 1150
  • Nishikai, M., Reichlin, M. (1980) Arthritis Rheum. 23, 881
  • Targoff, I.N. (1990) Rheum. Dis. Clin. North Am. 18, 455
  • Mathews, M.B., & Bernstein, R.M. (1983) Nature
  • 304, 177 Mirande, M. (1991) Prog. Nucl. Acid Res. 40, 95
  • Rosa, M.D. et al. (1983) Nucl. Acids Res. 11, 853
  • Biswas, T. et al. (1987) J. Immunol. Meth. 98, 235
  • Raben N. et al. (1991) Nucl. Acids Res. 20, 1075
  • Raben, N. et al. (1994) J. Biol. Chem. 269, 24277
  • Blechynden, L.M. et al. (1996) Gene 178, 151
  • Targoff, I.N., Reichlin, M. (1987) J. Immunol. 138, 2874
  • Walker, E.J. et al. (1987) J. Immunol. Meth. 96, 149
0

You might also be interested in ...

Anti-Calprotectin

Anti-Calprotectin

AMC04
Mouse Monoclonal antibody
Enquire
Sm

Sm

ATS02
Native - Calf Thymus
Enquire
Elastase

Elastase

ATE01
Native - Human Neutrophils
Enquire
PCNA

PCNA

ATP03
Recombinant
Enquire
AROTEC Diagnostics
AROTEC have been producing and supplying premium reagents to the diagnostic industry since our incorporation in 1996
Read More
   
Contact Us

info[at]arodia.com

+64 4569 0361

207 Gracefield Road,
Lower Hutt, 
Wellington