icon lock Secure Shipping Worldwide
cart Shopping Cart Cart (0)




Native - Calf Thymus
DNA topoisomerase I
Uniprot ID:  F1MN93
mRNA RefSeq:  NM_001206487
Protein RefSeq:  NP_001193416
Scleroderma (progressive systemic sclerosis) is a systemic autoimmune disease characterised by collagen deposition and connective tissue destruction of the skin, blood vessels and certain internal organs. The prevalence of scleroderma has been estimated to be 1 per 5000. Approximately 20-28% of scleroderma patients have autoantibodies to a nuclear protein referred to as Scl-70. Anti-Scl-70 antibodies are found with a greater frequency in systemic sclerosis patients with proximal skin involvement than in systemic sclerosis patients with limited skin involvement.

The molecular weight of enzymatically-active topoisomerase I determined by SDS-electrophoresis has been reported to range from 67-100 kDa. Recent data suggest that the 100 kDa form is the predominant form in vivo, and that the lower molecular weight forms are due to proteolytic degradation. Both the native and lower molecular weight forms have been shown to effectively bind anti-Scl-70 autoantibodies.

The amino acid sequence of human Scl-70 antigen (DNA topoisomerase I) has recently been described after cloning and sequencing its cDNA. The deduced molecular weight of the mature protein is 90,649 kDa; lower than the SDS-electrophoretic molecular weight. This may be due to post-translational processing or to anomalous electrophoretic behaviour of the protein. The protein was found to be very basic, with a calculated isoelectric point of 10.05, and to possess its catalytic activity within a 67.7 kDa C-terminal fragment. 

PDF-logo-dl Scl-70 datasheet
Please log in to view certificates of analysis for this item
  • Medsger, T.A. Jr. (1985) in Arthritis and Allied Conditions, 10thEd. (McCarty, D.J. ed.), pp 994-1036, Lea & Febiger, Philadelph.
  • Steen, V.D. & Medsger, T.A. Jr. (1990) Rheum. Dis. Clin. North Am. 16, 1
  • Douvas, A.S. et al. (1979) J. Biol. Chem. 254, 10514
  • Steen, V.D. et al. (1988) Arthritis Rheum. 31, 196
  • Shero, J.H. et al. (1986) Science 231, 737
  • Guldner, H. et al. (1986) Chromosoma 94, 132
  • Maul, G.G. et al. (1986) Proc. Natl. Acad. Sci. USA 83, 5145
  • Schmitt, B. et al. (1984) Eur. J. Biochem. 144, 127
  • Samuels, D.S. et al. (1994) Mol. Biol. Reports 19, 99
  • Hildebrandt, S. et al. (1990) Clin. Immunol. Immunopathol. 57, 399
  • D´Arpa, P. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 2543
  • Juarez, C. et al. (1988) Arthritis Rheum. 31, 108
  • Tsay, G. et al. (1990) J. Rheumatol. 17, 1314
  • Seelig, H.P. et al. (1993) J. Immunol. Methods 165, 241

You might also be interested in ...



Native - Bovine Heart Muscle
Parietal cell antigen (H/K-ATPase)

Parietal cell antigen (H/K-ATPase)

Native - Porcine Gastric Mucosa


Native - Calf Thymus
RNP-68K (Sm-Free)

RNP-68K (Sm-Free)

Native - Calf Thymus
AROTEC Diagnostics
AROTEC have been producing and supplying premium reagents to the diagnostic industry since our incorporation in 1996
Read More
Contact Us


+64 4569 0361

207 Gracefield Road,
Lower Hutt,